Protein Modification Reagents | Fisher Scientific (2024)

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Protein Modification Reagents | Fisher Scientific (1)

Chemical agents used to modify the amino acid side chains of proteins in order to alter their native charges, block or expose reactive binding sites, inactivate functional groups, and change functional groups to create targets for crosslinking and labeling.

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Protein Modification Reagents | Fisher Scientific (2)

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1

Thermo Scientific™Bond-Breaker™ TCEP Solution, Neutral pH

Protein Modification Reagents | Fisher Scientific (5)

Efficiently reduce protein disulfide bonds with this stable, odorless, 0.5M solution of TCEP reducing agent that is ideal for making reducing SDS-PAGE sample buffers.

2

Gibco™PNGase F Glycan Cleavage Kit

Protein Modification Reagents | Fisher Scientific (7)

PNGase F Glycan Cleavage Kit includes all components necessary to perform the enzymatic removal of almost all N-linked oligosaccharides from glycoproteins.

3

Thermo Scientific™8M Guanidine-HCl Solution

Protein Modification Reagents | Fisher Scientific (9)

Ready-to-use, 8M solution of guanidinium hydrochloride (GdnCl, GuHCl), CAS 50-01-1; dilute and pH-adjust to any concentration for protein biology methods.

Promotions are available

4

Thermo Scientific™Pierce™ TCEP-HCl, No-Weigh™ Format

Protein Modification Reagents | Fisher Scientific (11)

Reduce protein and peptide disulfide bonds using this thiol-free, pure, crystalline tris (2-carboxyethyl) phosphine hydrochloride, TCEP (CAS 5961-85-3).

Promotions are available

Form Solid

5

Thermo Scientific™DTNB (Ellman's Reagent) (5,5-dithio-bis-(2-nitrobenzoic acid)

Protein Modification Reagents | Fisher Scientific (13)

Measure peptide cysteines or other free, reduced sulfhydryls (-SH groups) in solution using this thiol-reactive, colored compound (Amax 412nm).

Promotions are available

6

Thermo Scientific™Pierce™ Alkylating Reagents

Protein Modification Reagents | Fisher Scientific (15)

Alkylating reagents provided in single-use, pre-weighed formats to streamline protein preparation workflows.

Promotions are available

Product Type Alkylating Reagent
Product Line Pierce™

7

Thermo Scientific™Pierce™ DTT (Dithiothreitol), No-Weigh™ Format

Protein Modification Reagents | Fisher Scientific (17)

Thermo Scientific™ Pierce™ DTT (Dithiothreitol) No-Weigh™ Format is designed for convenient preparation of stocks that will be added to protein samples for effective reduction of disulfide bonds.

Promotions are available

8

Thermo Scientific™Pierce™ 2-Mercaptoethanol

Protein Modification Reagents | Fisher Scientific (19)

Pure, liquid (equals 14M), beta-mercaptoethanol (bME, 2BME, 2-ME, b-mer), CAS 60-24-2; thiol reducing agent for cleaving protein disulfide bonds (cystines cysteines).

9

Thermo Scientific™Proteinase K Powder (≥30 U/mg)

Protein Modification Reagents | Fisher Scientific (21)

Cleave at the carboxyl-side of aliphatic, aromatic or hydrophobic residues and digest-inactivate DNase and RNase in nucleic acid purification using this protease.

10

Thermo Scientific™Pierce™ NEM (N-ethylmaleimide)

Protein Modification Reagents | Fisher Scientific (23)

Small, sulfhydryl-reactive (maleimide) compound to permanently block reduced cysteines of proteins or peptides to prevent disulfide bond formation.

Promotions are available

11

Thermo Scientific™Pierce™ HRV 3C Protease Solution Kit (2 units/μL)

Protein Modification Reagents | Fisher Scientific (25)

Remove fusion tags from proteins with the HRV 3C cleavage sequence with this recombinant cysteine protease that is dual tagged for easy removal from the sample after cleavage.

Protein Modification Reagents | Fisher Scientific (26)

Content And Storage Store at -20°C.
Product Type Protease
Form Kit
Product Line Pierce™

12

Thermo Scientific™Pierce™ Immobilized TCEP Disulfide Reducing Gel

Protein Modification Reagents | Fisher Scientific (28)

Efficiently reduce protein or peptide disulfide bonds with this stable, odorless, beaded agarose resin on which Tris (2-carboxyethyl) phosphine is immobilized.

Protein Modification Reagents | Fisher Scientific (29)

13

Thermo Scientific™Pierce™ DTT (Dithiothreitol)

Protein Modification Reagents | Fisher Scientific (31)

Reduce disulfides in biological buffers and sample loading buffers with pure, crystalline dithiothreitol (DTT), the most popular protein disulfide reducing agent.

14

Thermo Scientific™Immobilized Papain (Agarose Resin)

Protein Modification Reagents | Fisher Scientific (33)

Generate and purify Fab fragments from antibodies with cysteine-endopeptidase papain immobilized onto beaded agarose resin.

Protein Modification Reagents | Fisher Scientific (34)

15

Thermo Scientific™Immobilized Pepsin (Agarose Resin)

Protein Modification Reagents | Fisher Scientific (36)

Generate and purify Fab and F(ab')2 fragments from antibodies with acidic endopeptidase (pepsin) immobilized onto beaded agarose resin.

Protein Modification Reagents | Fisher Scientific (2024)
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